►
Description
In this video, Christian Silva, Founder and Chief Technology Officer of Fabrilab, gives an overview and demonstration of 3D bioprinting and how it may be applicable to the e-NABLE community in the future.
More information and discussion about EnableCon 2019 here: https://hub.e-nable.org/s/e-nablecon-2019/
More information about e-NABLE here: https://enablingthefuture.org
A
I'm
gonna
start
with
my
presentation,
so
I
ask
to
everybody
to
join
me
here.
I
wanna
explain
what
is
bioprinting
or
we
can
use
this
technology
in
enable
in
the
future
or
not
right
now,
because
we
need
to
learn
a
lot
of
stuff
before,
but
I'm
gonna
bring
the
basis
of
this
kind
of
technology
right.
So
that
is
my
main
propose
to
show
the
technology
will
show
the
biomaterials
and
what
I
expect
to
do
in
the
future.
With
this
technology
right
so
I
got
a
show.
A
This
is
a
technique
to
create
blood
vessels
with
different
kind
of
biomaterials
and
salts.
So
I
can
explain
later
why
I
was
focused
on
these
kind
of
tissues
and
how
we
can
use
these
technology
to
connect
practices
to
the
body.
So,
first
of
all,
here
in
the
States
more
than
21
people
is
dying
every
day
because
they
don't
have
a
way
to
get
an
organ.
A
That
is
a
huge
running
right
and
the
second
is
that,
normally,
when
you
are
doing
research
in
the
lab,
you
use
animals
like
these
pekes
I
come
out
like
a
monkeys
to
test
different
drugs
yeah,
and
that
is
something
that
is
going
against
the
I.
Don't
know
how
to
say
that
is
like
that:
the
ethics
exactly
yeah,
but
there
is
no
other
way
to
test
that
right.
So
we
would
like
to
use
this
kind
of
technology
to
test
drugs
and
all
kind
of
molecules
and
stop
chemicals.
A
Yes,
to
avoid
the
use
of
this
kind
of
experiment,
so,
first
all
I
gonna
spray.
What
is
3d
bioprinting
has
you.
You
can
see
in
the
same
world
by
all
means
now
use
you
can
use
biological
stuff
like
a
cells
right,
so
you
mix
the
clouds
with
biomaterials
to
get
a
bio
ink.
That
is
the
difference
between
the
normal
print
in
tree,
printing
and
the
bio
printing.
So
the
objective
of
this
is
to
guarantee
the
cell
function
a
by
ability
to
bring
constructs
that
define
a
scaffold.
A
So
one
of
the
most
important
things
of
the
bylanes
is
to
guarantee
the
diffusion
and
perfusion
of
media.
So
it
means
that
cells
can
get.
No
trains
can
get
media
to
feet
right
and
to
cook
to
get
connection
with
other
cells
right,
and
it
allows
the
use
of
different
kind
of
my
bio
matters
like
a
synthetics
like
material
that
replicate
the
extracellular
matrix
and
biologicals
biomaterials
as
well
like
a
cellulose
like
a
Junaid
right
to
jet
to
make
the
generation
of
dishes
and
analogous
and
organs
and
aloes
so
first
off
the
second
second.
A
So
3d
bioprinting
technology
has
six
steps.
The
first
step
is
to
take
the
imagine,
so
you
can
take
the
image
by
x-rays.
Ct
MRI
right,
usually
I
took
the
image
from
a
3d
scan
yeah.
So
the
second
step
is
the
design
approximation.
For
example,
if
you
take
a
3d
scan
from
the
earth,
you
have
the
digital
design
of
that
and
you
can
replicate
that
and
print
that
part.
As
you
know-
and
you
have
been
doing-
maybe
in
your
projects
right,
but
there
is
another
approximation,
not
only
biomimicry
is
cell
assembly
and
many
tissues.
A
You
can
use
that
approximation
to
prints
tissues
in
this.
In
the
third
step,
you
you
have
to
select
the
mat,
the
biomatter
land
you
are
going
to
use
like
siri
synthetic
polymers,
natural
polymers
material
that
can
replicate
the
cellular
matrix,
and
that
depends
on
the
kind
of
tissue
that
you
want
to
replicate.
No
all
the
biomaterials
are
biocompatible
with
all
kind
of
cells
and
know
all
the
biomaterial
you
can
implant
inside
of
your
body,
for
example,
if
you
use
one
kind
of
acrylate
to
make
a
cornea
or
something
like
that,
you
can
no
implant.
A
That
kind
of
armature
inside
of
your
knee,
because
it
is
going
to
create
an
a
reaction
right.
So
if
the
pants
of
the
kind
of
tissue
that
you,
then
you
can
select
the
cells
that
you're
gonna
use,
mix
it
with
the
biomat,
you
can
select
cells
that
are
already
different,
yet
pluripotent
cells
or
multiple
ten
cells.
A
You
can
use
as
well
inject
technology,
NASA,
assistive
technology,
the
LP
or
lesser.
Another
kind
of
technologies
are
able
to
be
used
to
print
different
biomarkers
and
the
last
is
the
application.
You
can
put
the
tissue
that
you
pray
in
a
metal,
Mitrich,
moderation
and
process.
You
can
implant
that,
or
you
can
put
that
in
a
bit
rope
in
vitro
testing
right
just
to
make
the
tissue
functional.
A
So
here
are
some
of
the
sample
that
you
can
print
and
it
depends
on
the
level
of
difficulty
to
print
it
right.
So
we
can
find
two-dimensional
tissue
like
a
skin
and
cartage.
Yeah.
Curtis
is
a
two
dimensional
tissue
because
it
doesn't
have
vascular
network.
In
the
second
category
we
can
print,
for
example,
tubular
structure,
hollow
tubes,
like
bands
like
our
T
bolts,
like
the
track
explained
in
the
third
category,
we
can
find
hold
on
tooth
structure
like
the
stomach
like.
B
A
A
You
can
find
a
lot
of
density
in
the
in
the
structure
and
a
lot
of
sauce
I
mean
the
density
of
the
tissue
is
higher
than
in
a
common
tissue
right,
and
the
amount
of
certainty
do
you.
Use
is
higher
than
the
normal
tissues,
so
that
is
the
complexity
and
keep
alive
those
all
those
cells
is
pretty
hard
is
because
you
want
to
you,
have
to
fit
those
those
cells
and
you
have
to
keep
them
alive.
So
that
is.
That
is
why
it
is
needed
to
create
a
vascular
network
to.
C
A
Them
alive
yeah,
so
here
are
some
application
in
the
real
world
and
where
you
can
use
3d
bioprinting
technology.
So
this
is
an
example
from
china
and
in
your
city
there,
research
lab.
They
made
an
experiment
with
five
children.
There
is
four,
but
in
paper
you
can
find
the
five
examples.
So
you
breathe,
they
schedule
the
ears
scaffold
right
and
you
see
the
cell,
they
the
sample
with
their
their
own
cells.
So
after
after
a
while,
you
can
implant
that
to
the
children
like
you
can
see
here
in
the
sample
right.
A
A
Here
you
see
that
say
it's
Kaiba
hee-hee-hee
world
with
an
obstruction
in
their
lungs,
so
he
couldn't
breathe
properly
and
they
pray
that
that
structure
right
with
a
biomaterial
that
is
biocompatible,
so
it
means
and
biodegradable
as
well.
So
it
means
that
with
the
time
they
biometric
disappear
with
the
time
right.
So
the
only
left
is
the
cells
than
go
through
the
structure
and
create
the
tissue
to
soup
to
to
bring
a
super
today
to
the
to
the
lung
right
here
the
bladders
are
seeded
with
cells
and
it
can
be
implanted.
A
It
is
a
world
that
was
developed
by
Anthony
Atala
in
the
wet
leg
forest
you
can
see
his
talks
he's
dead
metal
on
internet,
so
he
is
developing,
and
in
this
moment
they
you
can
bring
a
kidney,
a
full
organ
and
functional
organ
right,
so
researcher
people
they
say
then
more
or
less
you
fight
changers.
You
will
be
able
to
find
the
way
how
to
buy
this
kind
of
organs
and
to
be
in
plaid
implanted
into
the
body
right.
A
So
I
cannot
show
you
sort
of
my
work
that
I
made
in
the
Erlangen
University
and
in
the
selling
company.
The
still
the
company
to
create
this
kind
of
technology
with
a
bio
printer.
So
here
is
some
network.
Is
a
bus
to
that
network
with
different
biomarkers,
with
difference
and
loggers
and
different
size.
But
the
most
important
is
that
you
can
create
the
real
environment
of
the
cells
and
the
vascular
network
using
different
kind
of
cells.
A
At
the
same
time,
so
here
we
can
find,
for
example,
sending
primary
that
is
mix
it
with
power
glass
selling
laminate.
That
is
missing
with
endothelial
cells,
one
cord
that
is
made
with
pure
onyx.
This
is
such
a
special
kind
of
biomaterial
when
you
increase
the
temperature
it
becomes
top
and
when
you
decrease
the
temperature
it
becomes
liquid,
so
that
is
pretty
good
to
create
tissues
and
you
can
create
a
similar
matrix
with
cell
in
German.
So
there
is
poor
biometallic,
with
different
kind
of
that.
A
A
A
That
the
development
is
biocompatible
and
you
can
implant
them
that
on
humans
means
right,
so
that
structure
has
fiberglass
endothelial
cells
and
an
internal
lumen
that
was
made
it
back
with
pure
onyx,
so
I
decrease
the
temperature
and
then
I
profuse
a
media
to
the
vascular
network.
The
about
the
the
vascular
structure.
A
Yes,
to
show
that
if
the
structure
can
conduit
median
and
blood
as
well-
and
here
is
the
micro
fluidics
device
that
I
developed
just
to
show
that
the
disposable
as
well
to
print
a
structure
like
this
I
mean
normally,
the
microfluidic
structure
are
2d.
But
with
this
development
you
can
create
3d
structure
to
test
drugs
or
something
different.
So
with
this
much,
it
is
possible
to
make
the
optimization
of
the
construction
and
of
the
structure
that
you
want
to
test
with
microfluidic
technology.
D
A
The
state
of
the
art
of
this
kind
of
technology,
we
can
say
that
it
is
the
higher
level
of
the
research
in
this
kind
of
device.
So
if
you
can
connect
different
micro
organs
on
a
chip
right
with
vessels,
it
appears
this
magazine.
That
is
one
of
the
most
important
in
the
world
that
is
called
at
science
right
and
I
want
to
show
I
want
to
share
a
video
of
this
development.
E
F
A
A
And
they
need
for
vascular
recession.
So
one
of
the
biggest
challenge
that
is
facing
into
the
biofilter
field
is
to
develop
the
way
how
to
replicate
vascular
networks,
because
as
I
say,
that
is
the
only
way
to
keep
the
cells
alive
right
and
to
take
the
waste
products
from
the
cells
away
right.
That
is
why
nobody
is.
There
is
no
an
organ
that
is
able
to
be
transplanted
enough
in
a
human
being.
A
A
A
D
D
A
A
You
can
use
many
many
differents
biomaterials
to
be
implanted
into
the
body
with
those
kind
of
biomass
you
can
replicate
each
like
intestine,
liver,
pancreas.
With
conductive
ink
filings.
You
can
create
neural
tissue
models.
All
of
these
examples
is
for
research
proposed
it
is.
It
doesn't
mean
that
you
can
implant
that
end
up
into
the
body,
because
you
have
to
follow
a
low
hum
process
like
when
you
create
a
track.
A
Then
you
have
to
test
that
with
with
a
preclinical
testing
and
with
animals,
and
do
all
this
protocol
very
well
just
to
make
pretty
pressure
that
that
is
secure
for
people
right.
So
I,
don't
know
if
you
have
seen
on
the
news
heard.
That
was
like
a
pigeon
right,
something
like
this
inside
of
a
gel
that
is
the
fresh
print
in
approximation,
so
you
can
print
inside
of
material
that
serves
like,
like
a
structure
like
a
superbug
material.
You
can
print
that
and
make
it
works
right.
A
Of
the
the
the
printhead
and
change
the,
for
example,
for
the
temperature
control
for
the
shooting
Pam,
that
is
some
some
mechanically.
It
doesn't
use
our
pneumatic
system
or
you
can
use
the
inject
pre
head
or
the
thermoplastic
one.
So
it's
up
to
you
what
you
want
to
use
and,
of
course,
what
kind
of
biomaterial
you
want
to
create.
A
For
example,
I
want
to
say
for
Sorrell
that
an
arrow
working
with
saying
at
this
moment,
I
I,
was
interred
and
Selene
to
the
three
moons
in
Sweden,
but
I
I,
just
I
am
just
bring
in
information
to
you.
It's
up
to
you,
you,
you
want
to
use
the
technology
or
not
right
so,
but
I
just
want
to
show
you
what
is
the
characteristics
of
this
machine?
A
The
most
important
is,
for
example,
that
you
can
realize
the
the
ad
motor
inside
with
UV
and
as
well
with
the
epic
features
are
here
that
is
pre
important
at
the
moment
when
you
are
working
with
cells,
you
have
to
guarantee
the
sterilization
of
all
the
environment
as
well.
It
is
a
modular
system
that
can
control
the
temperature
and
the
chapter
2
as
well.
He
added
the
pre-health
in
the
print
bed.
D
A
F
A
A
H
A
That
molecule
is
bring
the
communication
between
the
South
like
aggregate
and
collagen
Matalin.
There
is
an
example,
but
there
is
many
many
many
things
that
happens
inside
of
a
tissue
so
and
you
have
to
guarantee
that
you
can
mix
different
biomaterials
at
the
same
time,
and
that
is,
there
is
no
slight
B
yeah,
for
example,
when
you
mix
PLA
with
ABS.
What
happens?
Tell
me
won't.
A
Ionic
method
to
cross
link
the
polymers
that
are
inside
of
the
structure
and
change
the
stiffness
of
the
structure
and,
for
example,
you
see
here,
wait
right.
It
doesn't
look
like
a
bone
and
using
different
cross-linking
ways
you
can
guarantee
that
structure
is,
is
quite
close
to
the
structure
of
bone
right,
yeah
so
and
the
most
important
you
have
to
current
in
the
integration
of
the
structure
that
you
clean
into
the
body
right.
A
So
silly
is
working
with
all
those
universities,
most
of
them
in
Europe
and
here
in
the
states
right
and
I
wanna
show
you
some
of
my
work
with
the
printer
that
I
assembly
by
myself
and
I
designed
the
free
world
they
had
were
the
electronics
and
the
pneumatic
system
to
make
it
possible.
So
how
do
you
see
to
see
here.
A
So
it
means
that
you
can
use
for
different
spree
heads
I'm,
pretty
different
biomaterials
mix
it
with
different
selves
and
put
all
together
to
create
for
the
sample
tissue
like
a
skin
right,
because
the
skin
is
not
just
a
single
layer.
A
skin
has
many
letters.
So
that's
why
it
is
important
to
have
this
technology
and
be
able
to
print
a
structure
with
difference
biomatter
here,
a
structure
that
can
be
used
for
my
micro
fluidics
proposed.
A
H
A
H
H
A
A
So,
sir,
sorry,
the
interview
I
developed,
the
coaxial,
no
soul,
just
to
print
a
structure
like
like
happens,
like
vascular
network
I.
Had
you
can
see
here
in
the
video
you
can
put
in?
At
the
same
time,
all
materials
in
the
concentric
way,
like
I,
say
a
Cox
a
way
for
each
of
the
material
in
a
single
way.
So
this
kind
of
notion
can
be
used
as
well
in
normal
printing
or
to
bring
forth
or
something
like
that.
It
is
not
the
only
application
of
that
is
known
only
by
printing.
J
A
D
A
A
J
A
Yeah,
so
you
have
to
wait
to
print.
For
example,
if
you
print
a
vascular
Network,
you
can
bring
with
the
biomarkers
and
aptitude
Asst
those
kind
of
cells
and
wait
just
for
the
immigration
of
those
cells
to
grow
through
that
biomatter
or
you
can
see
the
biomaterials
with
the
cells
and
accelerate
the
process.
F
A
Even
it
is
going
to
span,
but
is
to
connect
synthetic
systems
directly
to
your
body,
but
no
need
to
have
to
make
the
control
of
the
processes,
because
it
is
demonstrated
that
if
you
use
the
directly
connection
with
an
air
of
your
arm,
the
control
of
the
processes
is
awesome.
This
is
much
better
than
if
you
use
my
electric
systems
or
other
kind
of
system,
and
the
other
is
to
have
total
feedback.
I
mean
you
can
sense
pressure
temperature
yeah
with
this
kind
of
approach.
J
A
H
J
D
A
That
is
my
dream
and
I
know
that
here
is
people
who
know
know
who
doesn't
know
they
don't
know
my
job
right
with
practices,
so
I
just
want
to
show
what
we
have
doing
and
what
in
what
we
are
working
right
now
with
the
3d
printed
processes
right.
So,
as
you
know,
I
started
to
work
with
parenthesis
in
2009
with
my
cousin,
my
cousin.
He
lost
part
of
his
hand,
so
I
create
these
mechanics
to
bring
heel
back
the
movement
of
the
fingers.
A
It
is
quite
close
to
the
mechanics
that
is
that
is
used
for
the
X
finger.
Maybe
you
know
they.
Maybe
you
design
something
similar
to
the
X
finger
right,
so
that
is
quite
close
to
that
design.
An
approximation
and
I
published
that
in
a
Cuban
magazine
review
medical,
so
nowadays
we
have
fix
it
already
more
than
260
processes.
3D
printed
processes
to
children,
not
only
in
Colombia,
is
all
around
Latin
America.
A
A
Professional
here
is
Uriel
arte,
who
is
still
occupational
therapist
and
she
helped
us
with
all
the
protocols
that
we
follow
with
children's
teaching.
Then
the
technology
and
training
then
with
the
use
of
the
processes,
and
here
is
Catherine
Pinto,
who
is
psychologist
and
she
evaluate
all
the
children's
from
the
beginning
to
the
end
of
the
process
and
I'll
spray
important
to
follow
them
and
to
as
assistant
during
all
day,
all
the
time
that
they
are
growing
and
Fabien
Pedraza,
who
is
art,
assists
and
practices.
A
They
are
my
people,
they
are
my
team
and
I
I
super
the
work
of
every
lab
and
give
me
five
on
them.
Yeah
without
them,
anything
will
be
possible
like
the
word
and
we
are
doing
in
Colombia,
so
we
use
this
kind
of
3d
scanner
to
take
the
digital
model.
As
you
know,
we
use
these
kind
of
printers
that
we
build
by
our
own
yeah.
A
We
don't
have
money
to
buy
other
commercial
printers
and
we
evaluate
all
the
kids
with
visions
and
physiatrist
just
to
compare
the
use
of
the
parenthesis,
the
commercial
ones
with
the
3d
printed
processes
right,
that's
a
spray
important
and
those
are
the
kinds
of
practices
that
we
develop.
So
the
partial
hand,
the
the
that
is
the
promi
Matic
hand.
That
is
the
result
of
the
feedback
of
the
children's
that
they
should
just
ask
what
to
change
right.
We
started
with
a
speed
wood
design.
You
know
what
is
it
yeah
the
flexi
hand
right.
A
We
started
with
that
and
we
realized
that
we
we
could
improve
many
anything's
on
that
design
right.
So
that's
why
we
are
using
or
on
the
side
that
we
already
share
with
a
community
two
years
ago.
Oh
109
remember
what
they
are
not
good.
Sorry,
okay,
okay,
okay,
so
you
can
download
those
designs
from
the
givers
yeah.
What.
G
A
D
A
D
A
A
D
D
B
H
A
A
A
This
model
is
a
commercial
model
that
we
are
only
selling
to
hospital
there
in
Colombia,
but
it's
just
for
them,
no
for
more
people,
because
we
have
to
test
piercer
all
work.
What
is
the
security
of
the
adult
device
with
with
them?
And
the
hospital
allows
us
to
do
that?
Research
with
the
people
with
in
that
posture,
oh
yeah
and
the
last
one
that
is
or
last
release,
and
it
is
using
device
that
is
:.
B
A
If
you
can
improve
it,
software
and
a
system
has
a
touchscreen,
so
we
are
using
this
device
to
control
the
processes
right
but
as
well.
We
are
using
the
device
to
teach
to
children's
home
to
work
with
robotics
right,
so
the
devices
is
controlling
the
proteases
but
as
well.
The
device
serves
like
interface
to
teach
stab
Tecna
robotics
right,
so
we
are
performing
different
workshops
with
childrens
teaching
them
artificial
intelligence
Internet
of
Things
right.
A
A
I
A
As
I
say,
we,
we
know
that
the
core,
the
power
of
the
knowledge,
is
high.
So
that's
why
we
are
empowering
children's
with
knowledge.
That
is
why
we
perform
different
workshops,
teaching
them
how
to
design
with
Tinkercad
design
their
decoration
for
their
practices
with
different
workshop
teaching,
their
robotics
right
and
teaching
them
how
to
cook
with
their
own
practices.
The
most
important
for
their
children
is
to
let
them
know
how
to
use
their
practices
to
do
daily
activities,
and
that
I
think
that
is
the
most
important
thing.
A
When
you
bring
a
processes,
is
not
just
bring.
A
practices
is
to
follow
over
the
process
and
they
teach
them
how
to
work
with
the
processes
in
different
kind
of
activities,
and
that
guarantee
that
the
children
is
going
to
still
using
the
practices
for
longer,
because
normally
childrens
are
using
the
proteases
one
twice,
and
then
they
left
the
proteases,
and
there
is
something
that
is
true
is
true.
But
if
you
teach
them
how
to
use
the
processes
in
the
proper
way,
they
won't,
they
are
going
to
use
practices
in.
A
A
K
A
We
have
received
many
help
from
many
people,
but
of
course
that
is
not
enough.
With
the
amount
of
children
there
are
in
Colombia
in
the
need
of
one
practices,
because
you
know
we
have
suffered
over
fifty
years
of
conflict
between
different
groups
and
the
land
mines.
They
are
spread
everywhere,
right,
I'm,
the
only
dad
the
thing
is
that
they
spread
the
the
farm,
the.
A
A
D
A
This
is
called
the
fresh
printed
metal
and
you
can
print,
of
course,
without
a
super
material,
so
I'm
gonna,
clean
something
here
and
I
gonna
show
you
what
is
a
protocol
that
you
can
use
so,
first
of
all
you
choose.
Maybe
you
want
to
know
how
to
work
with
a
bio
filter.
You
can
come
here
because
there
is
no
screen
that
I
can
share
from
the
touch
screen
to
the
computer.
A
So
then
you
can
select
the
tool
that
you're
gonna
use
to
print
it
and
I'm
gonna
select
the
second
tool,
so
the
middle
one
yeah,
so
I'm
gonna
use
the
pneumatic
three
millimeters
right
and
you
can
see
the
nozzle
diameter
that
is
410
the
pressure
that
is
20
kilo,
Pascal's
and
the
velocity
right.
It
is
20
millimeters
per
second
and
the
material
that
you
are
using
this
in
this
material
is
called
selling
a
star,
and
that
is
for
demo
right
now.